Effect of pesticides on the population of Azospirillum sp. and on ammonification rate in two soils planted to groundnut (Arachis hypogaea L.)
نویسندگان
چکیده
Pesticides are usually applied simultaneously or serially for groundnut (Arachis hypogaea L.) crop protection. This type of pesticide application often leads to a combined contamination of pesticides and their residues in the soil. Pesticides used in groundnut cultivation – monocrotophos and chlorpyrifos, singly and in combination with mancozeb and carbendazim, respectively – were tested for their effects on the population of Azospirillum sp. and ammonification in two soils planted to groundnut in the Anantapur District of Andhra Pradesh, India. The size of the Azospirillum sp. population was initially low in both soils. Application of pesticides, singly and in combination up to 5.0 kg ha-1 , significantly increased the population of Azospirillum sp. after 7 and 14 days of incubation in vertisol soil, whereas in laterite soil, the application of pesticides in the same combination up to 2.5 kg ha-1 increased the population of Azospirillum sp. in similar conditions. Mineralization of peptone-nitrogen was profoundly increased when soil was treated with pesticides, singly or in combination, at 2.5 kg ha-1 in both soils. Vertisol soil that received monocrotophos and chlorpyrifos singly at 5.0 kg ha-1 showed a considerable increase in ammonification rate after 14 days of incubation. Due to the synergistic and additive interactions between pesticides and microorganisms, the population of Azospirillum sp. and the rate of ammonification increased at particular concentrations of pesticides (i.e 2.5 to 5.0 kg ha-1), but at higher concentrations (7.5 and 10.0 kg ha-1) the pesticides exerted antagonistic interactions on the population of Azospirillum sp. and ammonification. Resumen: Comúnmente se aplican pesticidas de forma simultánea o escalonada para proteger el cultivo del cacahuate o maní (Arachis hypogaea L.). Con frecuencia la aplicación de este tipo de pesticidas causa una contaminación combinada de pesticidas y sus residuos en el suelo. Se probaron los efectos de los pesticidas usados en el cultivo de maní –monocrotofos y clorpirifos, solos y en combinación con mancozeb y carbendazim, respectivamente– sobre la población de Azospirillum sp. y la amonificación en dos suelos plantados con maní en el Distrito Anantapur de Andhra Pradesh, India. El tamaño de la población de Azospirillum sp. era inicialmente bajo en ambos suelos. La aplicación de pesticidas –solos y en combinación– de hasta 5.0 kg ha-1 incrementó significativamente la población de Azospirillum sp. después de 7 y 14 días de incubación en suelo de vertisol, mientras que en suelo de laterita, la aplicación de pesticidas en la misma combinación y hasta de 2.5 kg ha-1 incrementó la población de Azospirillum sp. en condiciones similares. La mineralización del nitrógeno de la peptona se incrementó sensiblemente cuando el suelo fue tratado con pesticidas, solos o en combinación, a una concentración de 2.5 kg ha-1 en ambos suelos. El vertisol que recibió monocrotofos y clorpirifos solos en una concentración de 5.0 kg ha-1 mostró un incremento considerable en la tasa de amonificación después de 14 días de incubación. Debido a las interacciones sinérgicas y aditivas entre pesticidas y microorganismos, la población de Azospirillum sp. y la tasa de
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